作者:嘉美生物 發表時間:2012/2/26 閱讀次數:1836(次)
Enzyme-linked immunosorbent assay (ELISA）also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.
酶聯免疫吸附劑測定（enzyme linked immunosorbent assay，ELISA）